Genomics
Genome is the total genetic content of cells, the whole set of inherited nucleic acids of an organism made of DNA. Genomics encompasses various technologies used to discover
and characterize genes like understanding gene expression, gene function and the selection and validation of genes leading to the design of efficacious and specific drugs.
Gene Design and Synthesis
|
NanoBioServices is gives its customers the services of gene synthesis. We synthesize and clone genes in the vector of your choice. Gene optimization is done to achieve higher productivity and stability in the host. We also synthesize genes using sequence information provided by the customer and clone into one of our standard vectors - pUC based vectors or TA vectors. We then sequence both strands of the gene. We provide the following to the customer:
- Plasmid DNA
- Glycerol Culture
- Sequence of the gene
|
|
|
Genome or chromosome Walking
NanoBioServices offers isolation of unknown target sequences upstream or downstream of known sequences in genomic DNA, cDNA (total RNA), and clones (BAC, plasmids).
Here upstream or downstream genomic sequences can represent the flanking regions or TSS(translation start sites, along with other gene regulating sequences. |
|
|
Plasmid Purification
| We offer small and large scale isolation of plasmid and BAC DNA, suitable for Southern Blotting, PCR and genotyping |
|
|
- Nucleic Acid Extraction and Purification
DNA Extraction:
NanoBioServices offers cost-effective HTP and HMW DNA Extraction from plants and animal tissues.
Genomics DNA Purification:
We offer purification of high molecular weight DNA from different tissues (rich in secondary metabolites and polyphenolic compounds) of various plants and animals.
Plants: Corn, Soybean, Cotton, Tomato, Sunflower, etc. Different tissues - seeds, leaves, roots, etc.
- Plasmid DNA Purification:
We also offer small and large scale isolation of plasmid and BAC DNA, suitable for Southern Blotting, PCR and genotyping.
|
|
|
Computational Genomics
- Compare DNA and protein sequences to public and internal databases
- Align multiple sequences and analyze gene evolutionary relationships
- Determine DNA and protein motifs
- Select PCR primers
- Assemble sequencing fragments
- Construct vectors
Real-Time QPCR Platform
NanoBioServices’ QPCR services are built upon a state-of-the-art technology platform. Our QPCR system detects and quantitates nucleic acid sequences in a high-throughput mode suitable for the requirements of today's drug discovery process. Using these systems we can accommodate any real-time QPCR need with standard or customized TaqMan® assays.
We offer :
- Residual DNA testing
- Biodistribution studies
- Genetic stability testing
- Custom QPCR assay development
- Custom QPCR assay validation
- High throughput capacity
- Research and Regulated services
Southern Blotting Platform
As part of its genetic stability testing panel, NanoBioServices offers the traditional Southern blotting technique. A probe specific to the gene of interest is generated by PCR amplification or restriction digestion, then radioactively labeled. Total DNA is extracted from the test articles and host cells using organic extraction methods, then digested with appropriate restriction enzymes that cut predictively within the expression cassette and randomly within the host genome. Restriction fragments are electrophoresed on agarose gel, denatured, transferred to membrane, and hybridized to radiolabeled probe. Band densities are visualized using an Alpha imager and quantitated using spot densitometry.
Cogenics’ Southern blotting platform services include:
- Confirmation of structure maps
- Determination of number of integration sites
- Analysis of construct copy numbers
- Sensitivity down to 0.1 pg target DNA
- DNA Sequencing
- mRNA Sequencing
- Northern Blot Analysis
- Southern Blot Analysis
- QPCR
- Residual DNA testing
|
- Phage Detection
- Marker Retention
- DNA Sequencing
- Southern Blot Analysis
- QPCR
- Residual DNA testing
|
custom molecular biology services:
- Subcloning
- PCR & PCR product purification
- RT-PCR
- DNA/RNA extraction
- Southern blot analysis
|
- Library screening
- Transformation
- Large scale plasmid DNA preparation
- Shotgun library construction (BAC, PAC, cosmid, genome)
|
Benefits :
- Superior quality.
- Flexible, tailored service projects.
- Excellent service for small, as well as large, projects.
High quality Low cost for all your research needs.
Applications of Genomics :
Molecular Medicine: lead to improved diagnosis of disease; early detection of genetic predispositions to disease; rational drug design; gene therapy and control systems for drugs; and
pharmacogenomics “custom drugs.”
Microbial Genomics: explore new energy sources (biofuels); environmental monitoring to detect pollutants; and safe, efficient toxicwaste cleanup.
Comparative Genomics: Comparative genomics involves the comparison of genomes where similarity between the genomes of two organisms is determined to gain additional insights about the composition of genomes and regions of similarity.
It also can be helpful in knowing:
How genes and genomics have evolved, how any two or more organisms or genes are related. It can enable us to gain valuable insights about gene expression, gene regulation and how to interfere it with the model organisms or humans. |
