Peptide Design
Peptide design has its own set of parameters and properties.
Most peptide antigens requested range in length from 12 to 16 residues and are relatively easy to synthesize. Peptides that are 9 residues or shorter are effective antigens, but peptides longer than 12 to 16 amino acids may contain several epitopes.
Peptides in excess of 18 to 20 residues begin to present more synthetic challenges.
Hydrophobicity
When designing your peptide, be aware that residues such as Ala, Cys, Ile, Leu, Met, Phe, and Val will increase the chance of the peptide having solubility problems. Keep hydrophobic residues below 50%.
Sequence-specific issues
Asp-Pro sequences or stretches of amino acids that require bulky protecting groups on their side chains during synthesis are problematic in solid-phase peptide synthesis or cleavage. These sequences can be avoided.
Peptide Design Service
NanoBioServices offer a peptide design service to help you design the optimal peptide for the antibody you need for your experiments.
Minimize solubility problems and maximize your chances of obtaining the antibody you need.
To accelerate your research as efficiently as possible, you need reliable, professional expertise combined with advanced technology. NanoBioServices offers its clients quality services in this competitive environment. Our experts employ sophisticated, proprietary techniques to express, purify, and characterize proteins that match your exact needs:
- Structure/Function Studies
- Activity Assays
- X-ray Crystallography
- NMR (metabolic labelling)
- Protein-protein interactions
- High-Throughput Screening (HTS)
Our professional protein specialists are ready to assist you in determining the expression system, construct, and purification procedure that is right for you.
Protein characterization
Upon project completion, our specialists assess the final product according to your specifications. Typically, this assessment includes any or all of the following:
- Electrophoresis
- Protein concentration determinations
- Western blotting
- Absorption spectrum
- N-terminal sequencing
- Mass spectrometry
- NMR spectroscopy
- Activity assay
Additionally, we will custom-dispense the final product in the quantities and packaging that best meet your needs
Proteomics computational
Proteomics can be defined as the qualitative and quantitative comparision of proteomes under different conditions to further unravel biological processes. It involves analysis of complete complements of proteins.
Includes- identification and quantification of proteins, its location, modifications, interactions, activities and ultimately their function.
1. Functional Proteomics
• Gene expression, proteins-proteins interactions.
• Protein folding.
• Structure-function relationship.
2. Comparitive Proteomics
Comparing proteome of two different organisms for functional and structural studies.
Eg: C.elgans proteome was used as an alignment template to assist in novel human gene identification.
Applications of Genomics and Proteomics
1. Better way to fight infectious diseases.
2. To understand the molecular mechanism of disease.
3. Understand the complex interplay of genetics and environmental factors.
4. DNA diagnosis and Drug designing.
5. To study gene–gene, gene–protein, protein–protein, and protein–ligand interactions.
6. Novel Proteins. |
